Publications
Below is a listing of current publications associated with use of VetMotl devices for sperm sample preparation in veterinary ART procedures. These publications are categorized according to the animal species and procedures involved.
Video Abstract: Simplified Approach to Frozen Semen Insemination in Mares
Dr. Lee Morris, Director, EquiBreed ART New Zealand
This presentation features research by Dr. Lee Morris on a simplified fixed-time AI protocol for mares using frozen semen, with a focus on the practical realities of equine reproductive management. Covering timing, semen longevity, and breeding efficiency, the video brings forward research that speaks directly to the needs of modern breeding programs.
For professionals working with VetMotl Sperm Separation Devices, the discussion reinforces the importance of precision, consistency, and workflow optimization in reproductive care. The result is a clear, research-based perspective on how better-timed protocols can strengthen breeding decisions and support improved outcomes in the field.
Jump to Species:
Equine
EQUINE: ICSI / Sperm Quality
Comparison of two sperm selection devices using variable sperm loads to enhance quality of sperm harvest from fresh-diluted stallion ejaculates.
Marcio Menezes Nunes, Maria Luiza Munhoz, Frederico Augusto de Souza Freitas, Maria Florencia Gallelli, Gustavo Ferrer Carneiro, Marcela Miragaya. Association for Applied Animal Andrology. Poster presentation in July 2024 at Association for Applied Animal Andrology Brazil. Information on file and available upon request.
Found VetMotl devices outperformed other sperm preparation systems in terms of consistency and quality, critical for reproductive clinics.
EQUINE: ICSI / Sperm Quality
Influence of VetMotl selection and choice of extender on stallion sperm quality stored at 50 degrees C for 24h.
Maria Luiza Munhoz, Marcio Menezes Nunes, Frederico Augusto de Souza Freitas, Maria Florencia Gallelli, Gustavo Ferrer Carneiro, Marcela Miragaya. Association for Applied Animal Andrology. Poster presentation July 2024 at Association for Applied Animal Andrology Brazil. Information on file and available upon request.
Demonstrated remarkable post-processing sperm viability, even after 24 hours at 50°C, showcasing the VetMotl device’s potential.
EQUINE: ICSI / Sperm Quality
Application of the microflow technique for the selection of stallion semen.
Bugno-Poniewierska M, Kochan J, Podstawski Z, Dilugosz B, Kij-Mitka B. Journal of Equine veterinary science, volume 125. June 2023. Anticipated publication in 2024.
Compared VetMotl device favorably to other sperm preparation methods, positioning it as the premium option for equine sperm sample quality.
EQUINE: ICSI / Sperm Quality
Sperm processing methods effectively reduce the DNA fragmentation index in samples with high initial values.
Paredes B, Hernandez-Aviles C, Ramirez-Agamez L, Favetta L, Ribeiro E, Amorim M, Neal M, Love C, Chenier T. Journal of Equine Veterinary Science, volume 125. June 2023. Anticipated publication in 2024.
Confirmed VetMotl device’s ability to reduce DNA fragmentation, ensuring genetic integrity for better pregnancy outcomes.
EQUINE: ICSI / Sperm Quality
Characterization of sperm cell membrane charge and selection of high-quality sperm using microfluidics in stallions.
Morgan F. Orsolini, Margo H. Verstraete, Machteld van Heule, Daniela Orellana a, Alyssa Ortega, Stuart Meyers, Pouya Dini. Department of Population Health and Reproduction, University of California, Davis, CA, USA. Theriogenology, August 2022.
Revealed that VetMotl-selected sperm possess superior membrane characteristics, reinforcing its role as a high-fidelity selection tool.
EQUINE: ICSI / Sperm Quality
Selection of frozen–thawed stallion semen by microfluidic technology.
Veronica Vigolo, Camille Gautier, Maria Elena Falomo, Christine Aurich. Reproduction in Domestic Animals, December 2022.
Confirmed VetMotl device’s ability to optimize sperm quality post-thaw, giving frozen equine semen new viability for IVF applications.
EQUINE: ICSI / Sperm Quality
Effect of microfluidic sperm sorting on equine ICSI blastocyst rate
E Lorenzen, R Sanchéz, J Spanner. VetEmbryo, Hellevad, Denmark. ISEET 2022.
Reported improved blastocyst formation in ICSI using VetMotl-selected sperm, demonstrating a direct performance advantage in embryo development.
EQUINE: ICSI / Sperm Quality
Use of microfluidics to sort stallion sperm for intracytoplasmic sperm injection.
Raul A. Gonzalez-Castro, Elaine M. Carnevale. Animal Reproduction Science, Volume 202, March 2019.
Validated VetMotl device’s potential in equine ICSI by significantly enhancing motility of selected sperm, highlighting its clinical value for advanced assisted reproduction.
EQUINE: IVF
Temporal characterization of conditions that promote functional capacitation of stallion sperm.
Joao D. de Agostini Losanoa, Jillian E. Guertina, Maura S. McGrawa, Lana Katza, Vilceu Bordignonc, Justin W. Callahama, Jose H.F. Pontesd, Perla Fleuryd, Marc Maseratid, Carly Turnere, Bradford W. Daigneaulta b 1 aDepartment of Animal Sciences, University of Florida, Gainesville, FL; bDepartment of Large Animal and Clinical Sciences, University of Florida, Gainesville, FL; cMcGill University, Department of Animal Science, Montreal, Canada; dIn Vitro Equinos, Mogi Mirim, Sao Paulo, Brazil; eLazy E Ranch, Guthrie, OK. March 2026.
VetMotl device use produced a more motile, more highly capacitated, and more acrosome-intact frozen–thawed stallion sperm population than swim-up, with superior kinematics, higher tyrosine phosphorylation rates, and better acrosome integrity across all time points evaluated.
EQUINE: IVF
Equine in vitro fertilization with frozen–thawed semen is associated with shortened pre-incubation time and modified capacitation-related changes.
Matheus Felix, Tamara Dobbie, Elizabeth Woodward, Renata Linardi, Carolina Okada, Rebeca Santos, Katrin Hinrichs. Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA, USA. Biology of Reproduction. March 2025.
VetMotl-selected sperm showed enhanced capacitation behavior, streamlining IVF workflows with better embryo development.
EQUINE: IVF
Time of sperm pre-incubation affects fertilization rates after standard IVF with frozen-thawed semen in the horse.
M. Felix and K. Hinrichs. Department of Clinical Studies, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, PA, USA. Reproduction, Fertility and Development 37(1). 2024.
Established the importance of pre-incubation timing with VetMotl-prepared sperm, directly impacting IVF fertilization success.
EQUINE: IVF
IVF with frozen-thawed sperm after prolonged capacitation yields comparable results to ICSI in horses: A morphokinetics study.
Soledad Martin-Pelaeza, Alejandro de la Fuentea, Kazuki Takahashia, Itzel Tirado Pereza, Jazmin Orozcoa, Carolina T.C. Okadab, Carlos Ramires Netoc, Stuart Meyersb, Pouya Dinia. Departments of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, USAa, Anatomy, Physiology, & Cell Biology, School of Veterinary Medicine, University of California, Davis, CA, USAb, GeneTech USA, Purcell, OK, USAc.
Proved that VetMotl-enabled IVF yields embryo development rates comparable to ICSI, positioning it as a lower-cost, high-value option.
EQUINE: IVF
Selection of frozen-thawed semen for standard in vitro fertilization.
Felix M, Hinrichs K. Journal of Equine Veterinary Science, volume 125. June 2023.76. Anticipated publication in 2024.
Showed that VetMotl-treated semen can unlock consistent results in equine IVF, bridging the gap with ICSI outcomes.
EQUINE: Low-Dose AI
A simplified fixed-time insemination protocol using frozen–thawed stallion spermatozoa stored at 17 degrees C for up to 24h before insemination.
Lee Morris, Ria Harteveld, Zamira Gibb. Equine Veterinary Journal. April 2024.
Demonstrated VetMotl device’s compatibility with simplified AI protocols, supporting its ease-of-use for field veterinarians handling frozen semen.
Bovine
BOVINE: IVF
The use of microfluidic sperm sorting on bovine frozen-thawed semen for in vitro fertilization.
Bruna Lopes Cardoso1,2, Mayra Fernanda Alves Borges2, Luiz Felipe Fachin Pazianoto3,2 Luísa Anastácia Santos de Oliveira2, Felipe Perein1. 1Universidade de São Paulo (FZEA), 2CPEX Embriões, 3Universidade de São Paulo – Faculdade de Medicina Veterinária e Zooctecnia. August 2024. Information on file and available upon request.
Demonstrated that the VetMotl device enhances IVF success using frozen-thawed bovine semen, ideal for commercial embryo production labs.
BOVINE: IVF
Use of a sperm separation device as an alternative for sires with low outcomes using gradient selection for in vitro embryo production: preliminary results.
Marcelo Cunha Xavier1,2, Maurício Antonio Silva Peixer1, Luiz Gustavo Araújo Oliveira1, Patrícia Furtado Malard2, Rodrigo Moura Martins3, Rosangela Vieira Andrade2, Joao Henrique Moreira Viana4,1.1Bio Biotecnologia da Reprodução Animal, Brasília, DF, Brasil; 2Universidade Católica de Brasília, Brasília, DF, Brasil; 3Universidade de Brasília, Brasília, DF, Brasil; 4Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brasil. August 2024 SBTE poster presentation. Information on file and available upon request.
Showcased the VetMotl device as a rescue tool for sires with low success rates in conventional gradients, offering a path to genetic utilization and revenue recovery.
BOVINE: IVF
Effect of bovine spermatozoa preparation on embryonic development in vitro.
Emma Lorenzen. VetEmbryo, Hellevad Denmark. April 2022. Information on file and available upon request.
Highlighted improved embryo development when bovine sperm was pre-processed with the VetMotl device, unlocking greater success in in vitro embryo production.